摘要
为明确超数排卵过程中机体血清代谢物的变化,以寻找与卵泡及卵子成熟相关的代谢物,研究利用液相色谱结合质谱(LC-MS/MS)的非靶向代谢组学方法,对同期发情后外源FSH注射前(1st)与人工输精前(2nd)供体绵羊血清中的代谢物进行分析.结果显示:共检测到1 158种代谢产物(正离子模式下721种,负离子模式下437种),注释表明代谢物种类最多的是脂类分子,其次是有机酸及其衍生物.根据VIP＞1;FC＞1.2或FC＜0.83和P＜0.05筛选,2nd与1st差异表达代谢物共295个,其中,上调代谢物186个,下调代谢物109个.差异代谢物功能分析共富集到88条功能通路,包括类固醇激素生物合成、花生四烯酸代谢、皮质醇合成和分泌等.进一步分析表明,血清中高含量的脱氧皮质酮、磷脂酰胆碱和花生四烯酸可能有助于绵羊超数排卵过程中卵泡的发育.研究结果为超数排卵中卵泡发育调控机制解析提供一定帮助,为超数排卵过程中的营养调控和供体选择提供参考.
Abstract
The object of this study is to investigate the changes of serum metabolites during multiple ovulation in sheep and look for metabolites related to follicular maturation.In this study,the untargeted metabolomics analysis was processed through LC-MS/MS to analyze the metabolites in the serum of donor sheep after simultaneous oestrus,before FSH injection(1st)and before artificial insemi-nation(AI)(2nd).The metabolomics analysis showed that 1 158 metabolites(721 in positive iron mode and 437 in negative iron mode)were detected and 617 were annotated.The diferential metabolites were screened based on the following criteria:VIP≥ 1;FC＞1.2 or＜0.83;P≤0.05.A total of 295 metabolites were differentially expressed between 2nd and 1st(186 up-regulated and 109 down-regulated).KEGG pathway enrichment showed that 88 functional pathways were enriched in IE groups,including steroid hormone bio-synthesis,arachidonic acid metabolism and fatty acid biosynthesis.This study showed that high content of deoxycorticosterone,phos-phatidylcholine and arachidonic acid in serum would be helpful for follicular developing in multiple ovulation.The results would help to explain the relation between blood material and follicular development and provide a theoretical basis for the nutrition regulation and do-nor selection in multiple ovulation.
维普
万方数据