生物学杂志2024,Vol.41Issue(1) :123-125.DOI:10.3969/j.issn.2095-1736.2024.01.123

一种适用于实验教学的果蝇DNA提取新方法

A new method to extract DNA from Drosophila melanogaster suitable for experimental teaching

魏远 王宏刚 朱玉山 李艳君
生物学杂志2024,Vol.41Issue(1) :123-125.DOI:10.3969/j.issn.2095-1736.2024.01.123

一种适用于实验教学的果蝇DNA提取新方法

A new method to extract DNA from Drosophila melanogaster suitable for experimental teaching

魏远 1王宏刚 1朱玉山 1李艳君1
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作者信息

  • 1. 南开大学生物国家级实验教学示范中心,天津 300071
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摘要

南开大学遗传学实验课程组将科研中常用的动物组织DNA提取方法改造后用于实验教学,获得了 1种适用于实验教学的果蝇DNA提取新方法.该方法由碱裂解、酸中和、离心3个步骤组成,具有操作简单、时间短、成本低、效果稳定等特点,获得的DNA无论是浓度还是纯度均可满足后续PCR扩增的需要,符合实验教学的要求.该方法简化了果蝇DNA的提取流程,为与果蝇基因表达分析相关实验项目的改革奠定了基础,有助于提升实验教学效果,推动课程建设.

Abstract

The common animal tissue DNA extraction method has been modified in the genetics course at Nankai University.This no-vel method of extracting DNA from Drosophila melanogaster can be applied in scientific research as well as in experimental teaching scenarios.The method comprises three steps which are namely alkali cracking,acid neutralization,and centrifugation.The method is characteristically simple,quick,stable,and cost-effective.The concentration and purity of the extracted DNA enable subsequent PCR amplification and are also conducive to experimental teaching.This method has simplified the process of extracting DNA from Drosophi-la melanogaster and has laid a foundation for the improvement of experimental projects related to Drosophila melanogaster gene expres-sion analysis.In addition,it has helped to improve the experimental teaching effect and curriculum design.

关键词

果蝇/DNA提取/碱裂解法/遗传学实验/实验教学

Key words

Drosophila melanogaster/DNA extraction/alkaline lysis/genetic experiment/experimental teaching

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基金项目

2020年天津市高等学校本科教学质量与教学改革研究计划项目(B201005506)

出版年

2024
生物学杂志
合肥市科学技术协会

生物学杂志

CSTPCD北大核心
影响因子:0.554
ISSN:2095-1736
参考文献量17
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