Here,mainly through solution NMR experiments,we found that the mutant sequence of Thrombin Binding Aptamer(TBA-M)and human telomeric sequence htel3 self-fold into G-quadruplexes in sodium solution,respectively.Appealingly,these two well pre-folded G-quadruplexes can further reassemble into a new heteromolecular G-quadruplex complex(TBA-M/htel3)spontaneously through DNA strand displacement.The stoichiometric ratio between TBA-M and htel3 in the complex is identified as 1∶1.The last three guanosines(G14G15G16)from the 3′-end of TBA-M are involved in the formation of the G-quadruplex core of TBA-M/htel3 complex via Hoogsteen base pairing.In this paper,we reveal for the first time that two well pre-folded G-quadruplexes enable a further Hoogsteen pairing-based DNA strand displacement,in which the reaction process and molecular mechanism are also investigated primarily.Our find-ing expands the understanding of the interacting pattern and recognition mechanism between differently folded structures of nucleic acids.
关键词
重组装异分子间G-四链体/G-四链体之间的链置换/核磁共振/圆二色谱/DMS足迹实验
Key words
reassembly of heteromolecular G-quadruplex/strand displacement between G-quadruplexes/NMR/CD/DMS footprinting
维普
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