Carboxymethyl pachymaran prevented depressive behaviour induced by sub-chronic stress
The aim of the study is to investigate the protective effect of carboxymethyl pachymaran on depressive behaviour induced by sub-chronic stress.With the daily exposure to unpredictable mild stress for 2 consecutive weeks,carboxymethyl pachymaran was ga-vaged in mice at a daily dose of 0.1 g/kg,0.2 g/kg or 0.4 g/kg for the last 7 days of the modelling procedure.After the experiment,the composition of gut microbiota was investigated by 16S rRNA high-throughput sequencing,and the levels of inflammatory factors in serum were determined by ELISA,the content of short-chain fatty acids in feces was measured by GC-MS,and the depressive behav-iour was evaluated by open field test and elevated plus maze test and forced swim test.Compared with the model group,low and middle doses of carboxymethyl pachymaran groups improved depression-like behaviour,and changed the composition of gut microbiota with a decrease in the relative abundance of Gram-negative bacteria(P<0.05).In addition,the middle dose of carboxymethyl pachymaran could lower IL-2(P<0.05)and IL-6(P<0.01)levels in serum,and each dose of carboxymethyl pachymaran could down-regulate the levels of isovaleric acid and NE,5-HT and Ach through modulating Lactobacillus and Parabacteroides relative abundance,thereby re-ducing the release of IL-6.In conclusion,carboxymethyl pachymaran regulated gut microbiota composition,decreased the release of gut-derived isovaleric acid and NE,5-HT and Ach,and inhibited depression-associated inflammatory factors IL-6 and IL-2,thereby preventing depression development.
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