In order to investigate the effects of allyl isothiocyanate(AITC)on the synthesis of 5-HT in EC and the biological functions of EC,edible grade AITC were used to intervene with RIN-14B cells(rat pancreatic endocrine cell line),a cell model of EC.The cal-cium ion concentration in the cells was detected by FLUO-8 AM,the expression level of genes related to 5-HT synthesis was analyzed by qRT-PCR,the levels of 5-HT were determined by UPLC,and the transcriptome of AITC-treated RIN-14B cells was detected and analyzed by RNA-seq and bioinformatics.The results indicated that AITC caused an increase in intracellular calcium ion concentration through activation of TRPA1,while upregulated the expression of Tph1(tryptophan hydroxylase)and Ddc(5-hydroxytryptophan decar-boxylase).In addition,GO and KEGG functional enrichment analysis on AITC treated RIN-14B cells showed that AITC mainly regula-ted glutathione metabolism and relate pathways such as antioxidant and inflammatory regulation,suggesting that AITC may regulate in-testinal homeostasis by stimulating EC to promote glutathione metabolism,and also participate in inflammatory regulation of the intes-tine.The above results provided experimental data and research ideas for further study of the effects of AITC on EC and deeper biologi-cal functions of the intestine.
关键词
异硫氰酸烯丙酯/肠嗜铬细胞/血清素/RNA-seq/生物学功能
Key words
allyl isothiocyanate/enterochromaffin cells/5-HT/RNA-seq/biological function
维普
万方数据