Isolation,identification and whole genome analysis of a heparin-degrading bacterium
A bacterium species was isolated from fecal specimens of white-headed langur.This bacterium is non-spore-forming,yellow pigment producing,gram-negative and catalase-positive.It was identified as Sphingobacterium daejeonense NS6-1 based on 16S rRNA gene sequence.NS6-1 can't hydrolyze starch but is a heparin-degrading bacteria.The heparinase activity of NS6-1 was 46.60 U/mL,and can be increased to 107.29 U/mL in the present of 100 mmol/L Ca2+.Routine drug susceptibility test by K-B disk agar diffusion method showed that NS6-1 was resistant to kanamycin and gentamicin,while sensitive to rifampicin,tetracycline,polymyxin B,ofloxa-cin,cefoperazone and vancomycin.The genome was 4381042 bp in length with 37.21%overall GC content,including 3852 protein coding sequences.Functional annotation revealed a heparinase Ⅱ/Ⅲ-like protein in CAZy,a aminoglycoside resistance gene in CARD,a gene encoding catechol 1,2-dioxygenas which can catalyze hydrocarbon-related catabolic in oil.Six gene clusters related to secondary metabolite biosynthesis were predicted by using antiSMASH,of which two were identified as carotenoid synthesis gene clus-ters and one emulsan synthesis gene clusters.In summary,NS6-1 is a heparin-degrading bacterium,with potential for degradation aro-matic hydrocarbons in petroleum and carotenoid synthesis.The present results would lay a theoretical foundation for the further applica-tion of the isolated strain.
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维普
