Isolation,identification and characterization of a toluene-degrading bacterium
To solve the environmental pollution problem caused by toluene emission,a bacterial strain that can use toluene as a single carbon source was screened from the soil near a furniture factory and identified as Pseudomonas sp.M1 by morphological characteriza-tion and 16S rDNA analysis.The growth conditions of the strain were optimized by single-factor experiments,and the strain was exam-ined under optimal growth conditions for its ability to utilize different substrates.The growth conditions were optimized by single-factor experiments,and the effects of organic matter utilization and surfactant on the growth and degradation of the strain were investigated un-der optimal growth conditions.The results showed that strain M1 had strong tolerance to toluene,and the degradation rate reached 63.03%after 3 d at 30℃,pH 7.0 and toluene mass concentration of 200 mg/L.In addition to toluene,M1 can also use benzene and ethanol as carbon source and energy source,and can still maintain a certain amount of growth under the combined pollutants of ben-zene,toluene,ethylbenzene and xylene.The nonionic surfactant TritonX-100 is moderately biodegradable and has the best promotion effect on toluene degradation by strain.At 100 mg/L toluene mass concentration,the toluene degradation rate was 93.35%after the addition of TritonX-100 with 0.5 CMC.
toluenePseudomonas sp.isolation and identificationdegradation characteristicssurfactant
万方数据
维普
