AsmiR156:potential hub in drought memory molecular regulatory network of Agriophyllum squarrosum(L.)Moq.
In order to detect how miRNA-mRNA interaction participates in drought response and drought memory of A.squarrosum,the drought-sensitive ecotype AEX of Agriophyllum squarrosum was selected as the plant material,and transcriptome and sRNA sequencing were performed after three rounds of drought-rehydration treatments.A total of 341 and 7858 A.squarrosum miRNAs(DEMIs)and mRNAs(DEMs)were identified in response to drought stress,respectively.The DEMI-DEM regulatory network showed that 24 A.squarrosum DEMIs were involved in the regulation of 1423 DEMs,and these target genes were mainly involved in cell differ-entiation function.Two drought memory miRNAs(DMMIs),miR156 and miR6173,were identified,which regulated 11 and 6 drought memory genes(DMGs),respectively.Target genes for miR156 included three transcription factors(TFs):AsRWP-RK,AsC3H11,and AsWRKY33;the target genes for miR6173 included two GSTs(glutathione S-transferase).In previous study,AsC3H11 and AsWRKY33 participated in the A.squarrosum drought memory hierarchical regulatory network as TF molecular switches,so A.squarrosum miR156 could serve as a candidate hub for the"miRNA-transcription factor-functional gene"network.This study partially elucidated the mo-lecular regulatory basis of drought response and drought memory in A.squarrosum,which will provide a theoretical basis for the devel-opment of stress-tolerant genetic resources in desert plants.
万方数据
维普
