The aim of this study was to investigate the influence of auraptene(AUR)on food intake in mice and its mechanism.Mice were randomly divided to three groups including control group,2 μmol/kg AUR group and 1 μmol/kg AUR group.AUR was continu-ously given by oral gavage for 2 weeks to measure the cumulative food intake of the mice,the expression of insulin-like growth factors(IGFs)in serum,liver and hypothalamus,insulin-like growth factor-binding proteins(IGFBPs)in liver,proopiomelanocortin(POMC)in hypothalamus.The levels of mRNA and protein of carnitine palmitoyltransferase 1A(CPT1A)as a peroxisome proliferator-activated receptor α(PPARα)target gene were also tested.The results showed that AUR promoted food intake in mice in a concentration-de-pendent manner,inhibited the expression of POMC(P<0.01),significantly promoted the expression of IGFBP-1 in liver(P<0.01),and significantly reduced the content of IGF-1 in serum and hypothalamus(P<0.01),but did not effect on IGF-1 in liver(P>0.05).Mean-while,AUR significantly increased the expression of CPT1A in liver(P<0.01).In summary,these findings suggested that AUR could significantly promote food intake in mice.The main mechanism was that AUR promoted food intake in mice because of increasing the expression of IGFBP-1 in liver by PPARα activation,which bound the free IGF-1 in serum to decrease IGF-1 in hypothalamus and in-hibit the expression of POMC.
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