首页|菌藻生物膜反应器处理养猪废水机制研究

菌藻生物膜反应器处理养猪废水机制研究

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利用菌藻共生生物膜系统处理畜禽养殖废水,反应器是关键的工序和重要的限制因素.设计菌藻生物膜反应器运行处理养猪废水,通过调节进水氨氮质量浓度探究其对污染物去除效率及微藻生物量的影响,扫描电镜观察载体上菌藻生物膜的形成情况,然后利用高通量测序进一步探究微生物群落的变化,从而揭示菌藻生物膜污水处理系统的污染物去除规律和微生物响应机制.结果表明,当进水氨氮质量浓度为 200 mg/L时,氨氮的日去除速率最高可达到 35.60 mg/(L·d),并在载体上观察到大量微藻和细菌的团簇体,表明菌藻生物膜形成,优势菌属为Prevotella_9、Clostridium_sensu_stricto_1、Acinetobacter和Bifidobacterium;当进水氨氮质量浓度为 300 mg/L时,氨氮的日去除速率显著提高,最高可达到 45.70 mg/(L·d),但持续的高氨氮负荷会对菌藻生物膜造成一定冲击,优势菌属演替为Comamonas和Christensenellaceae_R-7_group.
Study on the mechanism of a biofilm reactor based on microalgal bacterial consortia for piggery wastewater treatment
The bio-reactor is a key process and important limiting factor during the treatment of livestock and poultry wastewater through microalgal bacterial biofilm.In this paper,a biofilm reactor was designed for microalgal bacterial consortia to treat piggery wastewater,and the influent ammonia nitrogen mass concentration was adjusted to explore its impact on nutrients removal and microal-gae biomass production.Moreover,scanning electron microscopy was used to observe the formation of microalgal bacterial biofilm on the carrier.High-throughput sequencing was also used for microbial community analysis.The aim of this study was to reveal the change regularities of nutrients removal and the response mechanism of microbial community of microalgal bacterial biofilm wastewater treatment with the fluctuation of influent ammonia.The results showed that the maximum daily removal rate of ammonia nitrogen could reach 35.60 mg/(L·d)when the influent ammonia nitrogen mass concentration was 200 mg/L,and the dominant bacte-rial genus was Prevotella_9,Clostridium_Sensu_Stricto_1,Acinetobacter and Bifidobacterium.Moreover,a large number of microalgal bacterial clusters were observed on carrier illustrating the formation of microalgal bacterial film.When the influent ammonia nitrogen mass concentration increased to 300 mg/L,the daily removal rate of ammonia nitrogen significantly increased reaching a maximum of 45.70 mg/(L·d).However,the sustained high ammonia nitrogen load made a certain impact on the microalgal bacterial biofilm,while the dominant bacterial genera turned to Comamonas and Christensenellaceae_R-7_Group.

microalgal bacterial consortiabiofilm reactorthe mass concentration of influent ammonia nitrogennutrients removalmicrobial community

陈恒源、方叶子、郑华宝、王敏

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浙江农林大学 环境与资源学院,杭州 311300

浙江农林大学 环境与资源学院浙江省土壤污染生物修复重点实验室,杭州 311300

菌藻共生体系 生物膜反应器 进水氨氮质量浓度 污染物去除 微生物群落

国家自然科学基金项目浙江省重点研发计划项目

322000842021C03190

2024

生物学杂志
合肥市科学技术协会

生物学杂志

CSTPCD北大核心
影响因子:0.554
ISSN:2095-1736
年,卷(期):2024.41(4)