首页|盐胁迫对桔梗生理生化指标及总皂苷积累的影响

盐胁迫对桔梗生理生化指标及总皂苷积累的影响

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为探究桔梗对盐胁迫的响应,以二年生桔梗为试验材料,通过盆栽的方式,设置对照组(CK,正常浇水)、低浓度盐胁迫(S1,200 mmol/L NaCl)、中浓度盐胁迫(S2,400 mmol/L NaCl)和高浓度盐胁迫(S3,600 mmol/L NaCl)等4 个处理组,测定不同浓度盐胁迫处理下桔梗幼苗的生物量、生理生化指标及总皂苷含量.结果表明:随处理时间的延长,盐胁迫会抑制桔梗的正常生长,降低其生物量,且随盐胁迫浓度的增加抑制作用越显著;S1 有利于促进桔梗叶绿素含量的增加,而S2 及S3 均使叶绿素含量降低;叶片相对含水量随盐胁迫浓度的增加呈下降趋势;桔梗叶片及根部抗氧化酶活性均在重度盐胁迫和处理第 30 天时显著降低;桔梗叶片和根部脯氨酸含量、丙二醛(MDA)含量和总皂苷含量均随盐胁迫浓度及处理时间的增加而显著升高.研究可为改善盐渍土环境下桔梗的生长发育以及提高产量和品质提供理论依据,以期为桔梗在盐碱地种植提供一定参考.
Effects of salt stress on physiological and biochemical indicators and total saponin accumulation of Platycodon grandiflorus(Jacq.)A.DC.
In order to study the effect of salt stress on the growth and development of Platycodon grandiflorus,the experiment was conducted with two-year-old Platycodon grandiflorus as test material,four treatment groups including control(CK,normal watering),low concentration salt stress(S1,200 mmol/L NaCl),medium concentration salt stress(S2,400 mmol/L NaCl)and high concentration salt stress(S3,600 mmol/L NaCl)were set up,the biomass,physiological and biochemical indexes and total saponins content of Platy-codon grandiflorus seedlings were determined.The results showed that salt stress inhibited the growth of Platycodon grandiflorus and reduced its biomass with the increase of salt stress concentration,and S1 could promote the increase of chlorophyll content of Platyc-odon grandiflorus,S2 and S3 decreased the content of chlorophyll,the relative water content of leaves decreased with the increase of salt stress concentration,the activities of antioxidant enzymes in leaves and roots of Platycodon grandiflorus decreased significantly at S3 and the 30th day after treatment.The contents of proline,MDA and total saponins in leaves and roots of Platycodon grandiflorus in-creased significantly with the increase of salt stress concentration and treatment time.This study can provide a theoretical basis for im-proving the growth,yield and quality of Platycodon grandiflorus in salt-alkali soil.

Platycodon grandiflorussalt stressbiomassphysiological indicatorstotal saponin

张美茜、于娟、曹阳、麻剑南、包晨丽、王晓琴、贾鑫

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内蒙古医科大学 药学院,呼和浩特 010110

桔梗 盐胁迫 生物量 生理指标 总皂苷

国家自然科学基金地区科学基金项目

81960687

2024

生物学杂志
合肥市科学技术协会

生物学杂志

CSTPCD北大核心
影响因子:0.554
ISSN:2095-1736
年,卷(期):2024.41(4)