Study of pollen germination vigor and morphology in Actinidia deliciosa
In order to investigate the viability of kiwifruit pollen,and to study the morphological changes before and after pollen ger-mination,15 samples of Actinidia deliciosa pollens were used in this experiment.An in vitro culture method was used to determine pol-len germination viability,and scanning electron microscopy was used to observe the morphology of five female pollens and ten male pollens before and after germination.The results showed that the germination rate of pollens from male plants varied considerably.None of the five female pollens germinated.Among the 10 male pollen samples No.8 had the highest germination rate of 79.27%and No.9 had the lowest germination rate of 22.85%.The polar facial view of the 15 samples did not change before and after ex vivo incu-bation.These samples were all trilobate circles.However,there was a significant difference in their equatorial view.The pollen mor-phology did not change before and after the female plants were cultured in isolation,and they were all spheroidal.The pollens of the male plant were perprolate or prolate before germination,and after germination in culture in vitro,all 10 samples were sub-spherical,with the polar axis length becoming almost smaller,the equatorial axis length becoming larger,the P/E ratio becoming smaller,and the width of the germination groove becoming larger.The differences in the values of the morphological indexes between the samples were small.