Effects of trivalent chromium on fatty acid uptake and triacylglycerol synthesis in Saccharomyces cerevisiae
To study the effect and mechanism of trivalent chromium on lipid metabolism in saccharomyces cerevisiae,total lipid,fatty acids,and triacylglycerol content in yeast were detected by oil red O staining and thin layer chromatography.The number of lipid drop-lets in yeast cells was observed by inverted fluorescence microscopy,and the fatty acid composition in yeast cells was analyzed by GC-MS.The effect of trivalent chromium on yeast lipid content was observed.Real time quantitative PCR further detected the transcription of genes related to yeast lipid metabolism and analyzed the mechanism of changes in yeast lipid content.Finally,the functional conser-vation was verified in SMMC-7721 cells.The results indicated that when treated with 100 μmol/L trivalent chromium,the contents of total lipids,fatty acids and triacylglycerol were reduced,the number of lipid droplets was decreased in cells,and the composition of fat-ty acids was regulated in yeast cells.The transcription of fatty acid uptake genes FAA1 and FAA3 and triacylglycerol synthesis genes DGA1 and PAH1 was significantly down-regulated by trivalent chromium in yeast cells.The lipid content and transcription of fatty acid uptake genes ACSL1,ACSL3 and triglyceride synthesis gene DGAT2 was also reduced by trivalent chromium in SMMC-7721 cells.It is implied that trivalent chromium might control the lipid reduction by regulating the genes expressions involved in fatty acid uptake and triacylglycerol synthesis.
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