Effects of Lycium barbarum exosomes on proliferation inhibition and apoptosis of non-small cell lung cancer A549 cells
In order to clarify the effect of Lycium barbarum exosomes on proliferation inhibition and apoptosis of non-small cell lung cancer cell A549,the exosomes were extracted by ultra-fast centrifugation method.The morphology of exosomes was detected by transmission electron microscopy,and the diameter distribution of exosomes was detected by particle size analysis.A549 cells were treated with the extracted L.barbarum exosomes.The effect of L.barbarum exosomes on A549 cells proliferation was detected by CCK-8 method,EdU was used to detect the effect of L.barbarum exosomes on A549 cells,the migration efficiency of cells was detec-ted by cell scratch method and Transwell,the cloning ability of cells was detected by cell cloning method.The apoptosis rate of cells was detected by flow cytometry,Western Blot and qRT-PCR were used to detect the protein and gene expression of apoptosis pathway.The results showed that the extracted exosomes were saucer-like under electron microscope,and the size was 40-200 nm,which was consistent with the shape characteristics of the exosomes.Compared with control group,L.barbarum exosomes group could inhibit A549 cell proliferation in a concentration-dependent way(P＜0.01).The cell migration ability and cell cloning ability in different con-centrations of L.barbarum berry exosomes decreased in a concentration-dependent manner(P＜0.01),while the cell apoptosis rate in-creased in a concentration-dependent manner(P＜0.01).L.barbarum exosomes could up-regulate the pro-apoptotic factors such as Cl-Caspase3,p53 and Bax in a concentration dependent manner,and down-regulate the proteins and genes of anti-apoptotic factors such as Bcl-2(P＜0.01).The conclusion was that L.barbarum exosomes could inhibit the proliferation of A549 cells and promote the apoptosis of A549 cells,and L.barbarum exosomes could also induce apoptosis through mitochondrial pathway.
Lycium barbarumexosomenon-small cell lung cancer cellproliferation inhibitionapoptosis
万方数据
维普
