Study and analysis of root exudates of Lavandula angustifolia at different cultivation years
To explore the impact of different cultivation periods on the exudates of Lavandula angustifolia in Yili,Xinjiang,non-targe-ted metabolomic analysis was conducted by using liquid chromatography-mass spectrometry(LC-MS)on the rhizosphere soil samples of 1-year-old,3-year-old,and 5-year-old lavender plants(named L1,L3,and L5),as well as soil samples without lavender cultivation(L0).The results indicated that root exudates included nine main categories:organic acids,lipids,nucleic acids,carbohydrates,vitamins and cofactors,hormones,peptides,antibiotics,and steroids.Compared to L0,L1,L3,and L5 resulted in the identification of 26,17,and 21 significantly different exudates,respectively(P<0.001).Additionally,L1,L3,and L5 exhibited enrichment in 13,10,and 9 signifi-cantly different metabolic pathways(P<0.05),respectively.Compared to L1,L3 and L5 showed 27(P<0.05)and 9 significantly differ-ent exudates(P<0.001),respectively,and enrichment in 4 and 9 significantly different metabolic pathways(P<0.05),respectively.Fur-thermore,when compared to L3,L5 presented 13 significantly different exudates(P<0.05)and enrichment in 6 significantly different metabolic pathways(P<0.05).Overall,the exudates of Lavandula angustifolia root system underwent significant changes as the cultiva-tion period extended,with sterols initially decreasing and then increasing,while vitamins,peptides,and nucleotides gradually decreased.