Study on the tissue culture technique of Taihangia rupestris var.ciliata and the soil conditions for its domestication and transplanting
In order to solve the problem in natural reproduction,domestication and transplanting of endangered plant Taihangia rupes-tris var.ciliate,its tissue culture technique was studied.Furthermiore,based on the analysis of soil physicochemical properties in habitat of T.rupestris var.ciliate,the soil conditions for domestication and transplanting of its cultured planets were established.The results were as follows:moderately mature leaves and petioles were the suitable explants for callus induction easy to be sampled with slight sampling disturbance to wild T.rupestris var.ciliate.The explants were sterilized in 0.1％aqueous mercuric chloride for 12 minutes,and then,had the lowest pollution rate and the highest success rate.The results of orthogonal test showed that the optimal medium for-mula for callus induction was as follows:6-BA 0.1 mg/L+NAA 2.0 mg/L+2,4-D 0.5 mg/L(or without 2,4-D)+agar 0.7 g/L+sucrose 30 g/L,pH 5.8.The problem of explant browning in callus induction of T.rupestris var.ciliate was effectively solved by reducing the culture temperature to 20 ℃.Then,the medium formula MS+6-BA 1.0 mg/L+NAA 0.2 mg/L+agar 0.7 g/L+sucrose 30 g/L,pH 5.8,was the most appropriate for the differentiation of indefinite buds.And the rooting culture medium was 1/3 MS+IBA 0.5 mg/L+KT 0.15 mg/L+agar 0.7 g/L+sucrose 30 g/L,pH 5.8.Measurement results showed that the volume weight was 1.0-1.1 g/cm3,the pH was 7.7-7.8,and the total phosphorus(TP),organic matter and available manganese content of habitat soil were positively related to the growth of T.rupestris var.ciliate,according to which the suitable transplanting medium for the cultured plantlets was garden soil∶rice husk carbon∶organic fertilizer 2∶2∶1 with relative air humidity 80％-90％and moderate shading in the early stage of transplanting.Under this cultivation technique,the survival rate reached 42.53％,and the growth was good.
Taihangia rupestris var.ciliatatissue culturehabitat soilphysicochemical propertydomestication and transplanting
万方数据
维普
