为探究不同生长条件下地生兰根内微生物的分布情况,通过宏基因组测序技术分析不同生长条件下根内微生物群落差异,并对其进行潜在功能预测.结果表明,野生状态下地生兰根内微生物的丰富度和多样性均高于组培状态下,且野生地生兰根内微生物主要核心菌群为变形菌门(Proteobacteria)、放线菌门(Actinomycetota)和子囊菌门(Ascomycota),组培状态下的核心菌群为变形菌门、担子菌门(Basidiomycota)和病毒类(Viruses noname).此外,野生状态下和组培状态下兰科菌根真菌(orchid mycorrhiza fungi,OMF)的主要优势类群也存在显著差异.丛枝菌根真菌(arbuscular mycorrhizal fungi,AMF)在组培状态下相对丰度显著高于野生状态下.通过对2 种不同生长条件下根内微生物代谢功能进行预测,结果发现,野生状态下碳水化合物代谢(carbohydrate metabolism)、氨基酸代谢(amino acid metabolism)、能量代谢(energy metabolism)以及辅因子和维生素代谢(metabolism of cofactors and vita-mins)等功能基因较为丰富.该研究结果为地生兰的保育、组培苗菌根化培育和病虫害防治提供了理论依据.
Analysis of microbial diversity in the roots of terrestrial orchids in Yunnan Province
In order to investigate the distribution of microorganisms in the roots of terrestrial orchids under different growth condi-tions,the differences of microbial communities in the roots under different growth conditions were analyzed by metagenomic sequen-cing technology,and the potential functions of these microorganisms were predicted.The results showed that the abundance and diver-sity of microorganisms in the roots of wild orchids were higher than that of those ochids in the cultivated condition.The main groups of microorganisms in the roots of orchids in the wild condition were Proteobacteria,Actinobacteria,and Ascomycota,while those in the cultivated condition were Proteobacteria,Basidiomycota and Viruses noname.In addition,there were significant differences in the main dominant taxa of orchid mycorrhiza fungi(OMF)in the wild and in the cultivated condition.The relative abundance of arbuscular my-corrhizal fungi(AMF)was significantly higher in the cultivation than in the wild.By predicting the metabolic functions of microor-ganisms in the roots under two growth conditions,it was found that functional genes for carbohydrate metabolism,amino acid metabo-lism,energy metabolism and metabolism of cofactors and vitamins were more abundant in the wild condition.The results of this study provided a theoretical basis for the conservation,artificial mycorrhizalization seedling cultivation,and pest control of terrestrial orchids.
万方数据
维普
