Metabolomic analysis of postharvest blueberry fruits treated by Bacillus tequilensis KXF6501 fermentation
This experiment was implemented to investigate the differences of the metabolites in blueberry fruits after the application of Bacillus tequilensis fermentation.The resistance mechanism of postharvest blueberry fruits treated by B.tequilensis fermentation was ex-plored and the theoretical references for the application of B.tequilensis in blueberry fruits storage and preservation were provided.Blueberry fruits were treated with B.tequilensis fermentation,the lesion diameters were measured.Subsequently,non-targeted metabolo-mics techniques were used to analyze the metabolites of blueberry fruits treated with B.tequilensis fermentation.Then,the content of phenolic compounds and flavonoids in blueberry fruit was determined.The result showed that blueberry fruit lesion diameters were sig-nificantly reduced after treated with B.tequilensis fermentation.Based on non-targeted metabolomics techniques,77 pathways were an-notated,and a total of 27 significant differential metabolites were enriched in the 20 metabolic pathways with the highest correlation in the group after treated with B.tequilensis fermentation.Among them,six pathways with significant influence were listed as follows:bi-osynthesis of phenylpropanoids,flavone and flavonol biosynthesis,biosynthesis of siderophore group nonribosomal peptides,flavonoid biosynthesis,apoptosis and C5-Branched dibasic acid metabolism.And the content of phenolic compounds and flavonoids in blueberry fruit was higher than that in the control treatment,which was consistent with the results of metabolomics analysis.After the treated with B.tequilensis fermentation,secondary metabolites accumulated in blueberry fruit,which in turn increased the disease resistance of blue-berry fruits.
万方数据
维普
