Establishment of a tetraploid Citrus.junos cv.Ziyang orange rapid propagation system
Using tetraploid Citrus.junos cv.Ziyang orange bud and stem segments were used as ex-plants,different plant hormone combinations were optimized through micro cutting technology to establish an efficient rapid propagation system.The results shows that the germination proliferation is the best when MT medium with 1.0 mg/mL 6-BA +0.1 mg/mL KT and the proliferation rate is up to 91.67%and the prolifera-tion coefficient is 1.82 after 45 days.With stem segments as explants,MT medium with 1.0 mg/mL 6-BA + 1.0 mg/mL KT hads the best axillary bud differentiation,the axillary bud differentiation rate is 76.67%and proliferation coefficient is 1.22 after 45 days.The 1/2 MT medium with 2.0 mg/mL NAA +0.2%AC(acti-vated carbon)has the best effect on inducing rooting,with a rooting rate of 92.59%after 60 days.46 seed-lings are transplanted and domesticated with the result of 95.7%survival rate.With the root tip chromosome compression counting method,the tissue cultured seedlings obtained are tetraploid Citrus.junos cv.Ziyang orange.A high-efficiency sexual reproductive system for tetraploid Citrus.junos cv.Ziyang orange is built in this research,providing a reference for subsequent polyploid research and production practice.