Study on the preparation,antioxidant,and antitumor activities of Stropharia rugosoannulata polypeptides
[Objective]Stropharia rugosoannulata,an emerging edible mushrooms in recent years,has gained market attention for its exceptional nutritional value and various health benefits.However,the rapid growth in production has been accompanied by a lag in deep processing technologies and a lack of innovative product development,posing challenges to its industrial ad-vancement.This study aimed to optimize the preparation process of S.rugosoannulata polypeptides and evaluate their in vitro antioxidant and antitumor activities,thereby providing a scientific basis for its deep processing and product innovation.[Meth-ods]Using the degree of hydrolysis as the evaluation metric,the preparation process of polypeptides was optimized through pro-tease screening,single-factor experiments,and orthogonal testing.Ultrafiltration was employed to obtain three polypeptide fractions(M1:>10 kDa,M2:3～10 kDa,M3:<3 kDa).Antioxidant activity was evaluated via DPPH·and ABTS+·scav-enging rates,while antitumor activity was evaluated based on tumor cell proliferation inhibition rates.[Results]The optimal preparation conditions for S.rugosoannulata polypeptides using trypsin were established as follows:a solid-liquid ratio of 1∶20 g/mL,enzyme concentration of 4000 U/g,and enzymatic hydrolysis duration of 3 hours.Under these conditions,the degree of hydrolysis reached 28.3%,a threefold improvement compared to unoptimized conditions.The scavenging rates of DPPH·and ABTS+·radicals were 69.2%and 91.4%,respectively,surpassing previously reported values.The polypeptide fractions exhibited varying inhibitory effects on four tumor cell lines(cervical cancer cell HeLa,lung cancer cell NCI-H460,breast cancer cell BT549,and liver cancer cell HepG2),all in a dose-dependent manner.Among the fractions,M1 and M3 ex-hibited the most significant inhibitory effects on NCI-H460 lung cancer cell,while M2 primarily targeted HeLa cervical cancer cells.Notably,M3 achieved the highest inhibition rate of 30.28%on NCI-H460 at a concentration of 500 μg/mL.[Conclusion]The preparation process of S.rugosoannulata polypeptides using trypsin was successfully optimized.The resulting polypep-tides exhibited promising antioxidant and antitumor activities,highlighting their potential for industrial application and product development.
万方数据
维普
