Optimization of Extraction Process of Total Flavonoids from Bitter Bamboo Shoot Waste
This study aimed to extract total flavonoids from bitter bamboo shoot waste using the ultrasonic-assisted solvent extraction method.By evaluating the effect of different process conditions on the extraction rate,this study determined the optimal process conditions for the extraction of total flavonoids from bitter bamboo shoot waste.Taking the extraction amount of total flavonoids as the evaluation index,selecting ethanol concentration,solid-liquid ratio,extraction temperature,and extraction time as testing factors,using Minitab software for experimental design,establishing a response surface regression model,and performing response surface analysis to optimize conditions of the extraction process of total flavonoids from bitter bamboo shoot waste.Results showed that the optimal conditions for the ultrasonic-assisted solvent extraction of total flavonoids from bitter bamboo shoot waste were:ethanol concentration 95％,liquid-solid ratio 40 mL/g,temperature 60℃,and time 40 minutes.Under these conditions,the extraction amount of total flavonoids from bitter bamboo shoot waste can reach 9.68 mg/g.Comparing to the standard methods,such as"Determine of Total Flavonoids in Plant-origin Food(DB43T 476-2009)"and"Determine of Total Flavonoids in Food the Spectrophotometric Method(SZDB/Z 349-2019)",the optimal method proposed in this study can significantly increase the extraction amount of the total flavonoids by 22.69％～31.34％.
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