3种方法分离鉴定金黄色葡萄球菌
Isolation and identification of Staphylococcus aureus by three methods
沈锐 1马力 1严海元 1肖陈 2赵壮志1
作者信息
- 1. 重庆市永川食品药品检验所,重庆永川 402160
- 2. 重庆市黔江食品药品检验所,重庆黔江 409000
- 折叠
摘要
通过参加2023年中国食品药品检定研究院组织的NIFDC-PT-451金黄色葡萄球菌(Staphylococcus aureus)检测能力验证计划,采用3种快速分离鉴定金黄色葡萄球菌的方法,与国标方法比较,提高了检验检测效率.分别采用国标法、全自动酶联免疫荧光分析仪法和实时荧光PCR检测法对随机人工污染样品TC04510042、TC04510204进行金黄色葡萄球菌检验.TC04510042、TC04510204均检出金黄色葡萄球菌,3种方法结果一致.金黄色葡萄球菌采用国标法,同时辅以全自动酶联免疫荧光分析仪法和实时荧光PCR检测法,不仅降低了假阳性率,也缩短了检测时间.后2种方法可作为大量样品的前筛查及应急检验,对筛查中出现的阳性样本用国标法进行确认,提高了检验检测效率.
Abstract
By participating in the NIFDC-PT-451 Staphylococcus aureus detection capability validation program organized by National Institutes for Food and Drug Control in 2023,three rapid isolation and identification methods for Staphylococcus aureus were adopted,which improved the efficiency of inspection and detection compared with national standard methods.The methods used were national standard method,fully automated enzyme-linked immunofluorescence analyzer method,and real-time fluorescence PCR detection method for the detection of Staphylococcus aureus in randomly artificially contaminated samples TC04510042 and TC04510204.Staphylococcus aureus was detected in both TC04510042 and TC04510204,and the results of the three methods were consistent.The use of national standard method for Staphylococcus aureus,combined with automatic enzyme-linked immunosorbent assay and real-time fluorescence PCR detection,not only reduces the false positive rate but also shortens the detection time.The latter two methods can be used as pre screening and emergency testing for a large number of samples,and the positive samples appearing in the screening can be confirmed using the national standard method,which improves the efficiency of testing.
关键词
金黄色葡萄球菌/能力验证/鉴定Key words
Staphylococcus aureus/proficiency testing/identification引用本文复制引用
基金项目
重庆市永川区自然科学基金重点项目(2022ycjckx20022)
重庆市永川区自然科学基金重点项目(2023ycjckx20004)
出版年
2024
NETL
NSTL
万方数据