In order to solve the problems of difficult recovery and poor stability of β-glucanase in industrial application,the β-glucanase immobilization research was carried out.Sodium alginate and β-glucanase were mixed and dropped into calcium chloride solution for hardening,and then β-glucanase was immobilized by glutaraldehyde crosslinking.The optimum conditions of β-glucanase immobilization were obtained by single factor test and orthogonal test:30.0 g/L of sodium alginate,30.0 g/L of calcium chloride,0.04％of glutaraldehyde,hardening for 2 h and crosslinking for 40 min.The results of stability test showed that the thermal stability and acid-base stability of β-glucanase were significantly improved compared with those of free enzyme.Finally,after the immobilized β-glucanase was recycled for 3 times,it was found that the enzyme activity remained more than 85％,but after the fourth use,the activity decreased rapidly to 63％,so the operation stability needs to be improved.
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