To establish a method forsimultaneous determination of nine polyphenol compounds,including neochlorogenic acid,mulberroside A,chlorogenic acid,cryptochlorogenic acid,rutin,isoquercitrin,nicotiflorin,isochlorogenic acid C and kaempferol.High-performance liquid chromatography equipped with Shim-pack GIS C18 column(4.6 mm × 250 mm,5 μm)was used for polyphenols detection under the condition of 30℃ column temperature.Acetonitrile-0.2％formic acid aqueous solution was used as mobile phase.The flow rate is 1.0 ml·min-1,10 μL extract was injected.Polyphenols was detected at the 350 nm wavelength under the conditions of gradient elutionl.Nine polyphenols were absolutely separated within 85 minutes.The linearity ranges of neochlorogenic acid,mulberroside A,chlorogenic acid,cryptochlorogenic acid,rutin,isoquercitrin,nicotiflorin,isochlorogenic acid C and kaempferol were 0.0170～0.1068 mg/mL,0.0154～0.1155 mg/mL,0.0146～0.1097 mg/mL,0.0130～0.0977 mg/mL,0.0149～0.1114 mg/mL,0.0136～0.1020 mg/mL,0.0170～0.1273 mg/mL,0.0070～0.0527 mg/mL,0.0151～0.1131 mg/mL,0.0058～0.0435 mg/mL,respectively.And the r values range from 0.9991 to 0.9998.The average recoveries of nine polyphenols were from 93.00％to 100.51％.the method was proved to be accurate and reliable,and can be used for the quality control of mulberry leaves.
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万方数据
