Establish a diagnostic method for Enterohemorrhagic E.coli O157.The detection method of rfbE gene was established by Recombinase Aided Isothermal Amplification(RAA)and its performance was evaluated.The method can complete the amplification detection within 20 min,and has no cross reaction with 5 kinds standard strains.The sensitivity of plasmid is 10 copies/μL,and nucleic acid is 102 CFU/mL.The data were stable after 48 hours of 37 ℃ pressure.The method has good specificity,high sensitivity,good stability and fast detection speed,and can be used to detect with Enterohemorrhagic E.coli O157.
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