Research on the proliferation of Bifidobacterium longum in Sparassis polysaccharide nutrient solution
To explore the effect of polysaccharides from Hydrangea on the proliferation and growth of Bifidobacterium longum,the nutrient solution prepared by Sparassis polysaccharide instead of glucose in MRS broth medium was used to culture of Bifidobacillus longum.The nutrient solution containing Sparassis polysaccharide was prepared with concentration gradient of 0%~1.8%,7 concentration gradients formed.At the same time,MRS broth medium and PYG liquid medium were selected as control and reference,and a small amount and the same amount of Bifidobacillus longum were added to the above nine groups of media,them at the following time during fermentation culture Oh,12 h,24 h,36 h,48 h,72 h,the growth dynamics of Bifidobacterium was determine,and the proliferation of Bifidobacterium in each medium was investigated.The results showed that Sparassis polysaccharide could be decomposed and utilized by Bifidobacterium,although it was used slowly,there was a positive correlation between the active bacteria concentration of Bifidobacterium and the Sparassis polysaccharide concentration in medium.When the content of Sparassis polysaccharide was 0.3%,the peak concentration of live bacteria in fermentation broth could exceed 106 CFU/mL,and when the content of Sparassis polysaccharide was 1.0%,the peak concentration of live bacteria in fermentation broth was more than 107 CFU/mL,the advantage of which was that it could maintain close to the level of live bacteria for a long time,which could slow the decline of Bifidobacterium.This study provides a new material and method for liquid fermentation to obtain higher active bacteria of Bifidobacterium and maintain higher concentration of live bacteria of liquid products.