Role of TXNDC12 in growth and metastasis of colorectal cancer
Objective To explore the expression of TXNDC12 in colorectal cancer and its effect on proliferation,migration and apoptosis of colorectal cancer cells.Methods The expression of TXNDC12 in colorectal cancer and in colorectal cancer patients with lymph node metastasis were predicted using GEPIA and UALCAN online websites.Eight cases of colorectal cancer tissues and paracancerous tissues from the Affiliated Hospital of Yan'an University were selected to detect the protein expression level of TXNDC12 by Western blot.Sanger Box website was used to analyse the correlation between TXNDC12 expression and the immune infiltration of colon rectal cancer tissues.The protein and mRNA expression levels of TXNDC12 in NCM460,HT-29,RKO and HCT116 cells were detected by Western blot and RT-qPCR.TXNDC12 was knocked down in HT-29 and RKO cells using small interfering RNA(siRNA)technology.The cells were divided into three groups:blank control group(MOCK group),control group(NC group)and knockdown group(si-TXNDC12-2 group),and the proliferation ability,the migration ability and the apoptosis of colorectal cancer cells were detected by CCK-8 assay and clone formation assay,Wound healing assay,Hochest fluorescence staining and Western blot assay,respectively.Results GEPIA analysis and Western blot results of clinical pathological tissues showed that TXNDC12 expression was higher in colo-rectal cancer tissues than that in normal tissues(P<0.05).UALCAN analysis showed that the expression of TXNDC12 was elevated in colorectal cancer patients with lymph node metastasis,and Sanger Box website analysis showed that TXNDC12 was negatively correlated with the abundance of immune infiltration in colorectal cancer patients(P<0.001).Western blot and RT-qPCR results showed that TXNDC12 expression was increased in HT-29,RKO and HCT116 cells compared with NCM460 cells(P<0.05),and compared with NC group,the cell viability,the number of clone formation,and the migration distance of HT-29 and RKO cells in si-TXNDC12-2 group were decreased significantly after knockdown of TXNDC12(P<0.05),while the apoptosis was increased(P<0.05).Conclusion TXNDC12 is highly expressed in colorectal cancer and negatively correlated with immune infiltration in colorectal cancer patients.Knockdown of TXNDC12 can inhibit the cell proliferation and the migration,and promote the apoptosis of colorectal cancer cells.