Lycium barbarum polysaccharide promotes phagocytic function of HL-60 cells
Objective To investigate the effect of lycium barbarum polysaccharide(LBP)on phagocytosis of human neutrophil line HL-60 cells and its related mechanism.Methods HL-60 cells were stimulated with different concentrations(0,1,10,100,200,500 μg/mL)of LBP for 24 h,and the viability and the phagocytic function of HL-60 cells were detected by CCK-8 and flow cytometry,and the phosphorylation of Akt in HL-60 cells was detected by Western blot.HL-60 cells were divided into control group,LBP group and LBP+PI3K inhibitor(Wortmannin)group for the blocking experiment.HL-60 cells in LBP group were stimulated with LBP(200 µg/mL)alone for 24 h.In LBP+Wortmannin group,HL-60 cells were pre-stimulated with 1 μmol/L Wortmannin for 2 h,and then treated with 200 μg/mL LBP for 24 h.HL-60 cells were divided into control group and 200 μg/mL LBP group,and then flow cytometry was used to detect the expressions of CD36,CD204,CD209,macrophage receptor with collagenous structure(MARCO),Dectin,and other receptors.Results Different concentrations of LBP had no significant effect on the survival rate of HL-60 cells(P＞0.05).Compared with 0 µg/mL LBP,1-200 µg/mL LBP significantly increased the phagocytic rate of HL-60 cells(P＜0.01).Compared to control group,the expression of phosphorylated Akt increased in 200 μg/mL LBP group(P＜0.05).The PI3K blocking assay demon-strated that LBP+Wortmannin partially suppressed phagocytosis of E.coli by HL-60 cells induced by LBP(P＜0.05).Compared with control group,the expression level of the MARCO receptor in HL-60 cells was significantly increased in LBP group(P＜0.05),however,there were no significant changes in CD36,CD204,CD209 and Dectin between two groups(P＞0.05).Conclusion LBP promotes the engulfment of E.coli by HL-60 cells through the PI3K-Akt signaling pathway,which may be related to the upregulation of MARCO receptor expression in HL-60 cells.
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