Effect and mechanism of histone deacetylase inhibitor SAHA on liver injury in chronic intermittent hypoxia mice
Objective To explore the effect and its mechanism of histone deacetylase inhibitor SAHA on the expression of apoptotic protein Caspase-9/Caspase-3 and the liver injury in chronic intermittent hypoxia mice.Methods Male C57 mice were randomly divided into normal group(control group),chronic intermittent hypoxia group(CIH group)and CIH+SAHA group.The mice in CIH+SAHA group and CIH group were treated with 8 h intermittent hypoxia every day in hypoxia chamber for four weeks.Starting from the 3rd week,the experimental mice were intraperitoneally injected with 50 mg/(kg·d)SAHA every day before the intermittent hypoxia for two weeks.After the 4th week of the experiment,the weight of mice was recorded,and then the mice were killed to observe the liver histopathological changes by HE staining,and detect the levels of ALT and AST in serum.Western blot was used to detect the protein levels of c-Caspase-3 and Caspase-9 in mouse liver tissue,and TUNEL was used to detect the cell apoptosis in mouse liver tissue.Results Compared with control group,liver index and liver tissue injury were significantly increased after 4 weeks of intermittent hypoxia in CIH group,the damage of liver tissues was found,and the expression levels of Caspase-9 and c-Caspase-3 proteins,and the cell apoptosis in liver tissue were significantly increased(P＜0.05).Compared with CIH group,liver index,ALT,AST,Caspase-9 and c-Caspase-3 protein expression levels and apoptosis index of liver tissue were decreased in CIH+SAHA group(P＜0.05).Conclusion SAHA may ameliorate the liver injury induced by chronic intermittent hypoxia in mice by inhibiting apoptosis-related protein Caspase-9/Caspase-3.
万方数据
维普
