Evaluation of composite modeling method for chronic atrophic gastritis and precancerous lesions of gastric cancer
Objective To evaluate the composite modeling method for constructing a rat model of chronic atrophic gastritis(CAG)or precancerous lesions of gastric cancer(PLGC).Methods Sixty SPF grade male SD rats were randomly divided into a control group(n=10)and a model group(n=50).The rats in control group were fed normally,while the CAG rats in model group were established with a composite modeling method using N-methyl-N'-nitro-N-nitrosoguanidine(MNNG)for free drinking,sodium salicylate and sodium deoxycholate for intragastric administration every other day,and irregular fasting.The treatment lasted 6 months.At the 3rd,4th,5th,and 6th month of modeling,the pathological degree of the gastric mucosa was compared between control group and model group.After modeling,rat blood was collected to detect the serum levels of pepsinogen Ⅰ(PG Ⅰ),pepsinogen Ⅱ(PG Ⅱ),and gastrin 17(G-17)by ELISA.Results The rats in model group showed atrophy of gastric glands at month 4,fibrosis at month 5,disappearance of gastric glands and atypical hyperplasia at month 6,and one rat developed cancer,with a mortality rate of 12％.Compared with control group,serum PG Ⅰ was increased(P＜0.05),PG Ⅰ/PG Ⅱ(PG R)was decreased(P＜0.05),and G-17 was decreased in in model group(P＜0.05).Conclusion The composite modeling method shows a high successful rate to prepare the rat CAG model,and the results are stable,but the preparation time is relatively long.Due to the individual difference,the modeling process of rats is difficult to completely unify.All rats develop gastric mucosal atrophy after composite modeling treatment,some rats develop PLGC,and a few rats develop gastric cancer.
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维普
