Effect of CeO2 nanoenzyme on hair growth of cyclophosphamide-induced alopecia areata mouse model
Objective To investigate the effect of CeO2 nanoenzyme on the hair growth of cyclophosphamide-induced alopecia areata mice.Methods Thirty female C57BL/6J mice were randomly divided into blank group,model group and CeO2 nanoenzyme(300 μg/mL)group,with 10 mice in each group.Mice in model group and CeO2 nanoenzyme group were intraperitoneally injected with cyclophosphamide once to establish the model of alopecia areata.Mice in CeO2 nanoenzyme group were coated with 300 pg/mL CeO2 nanoenzyme on the back shaving area of mice,and mice in blank control group and model group were coated with equal volume of PBS solution once a day for 27 d.After the experiment(at day 28),the hair growth in the bald area of the back of mice was observed and scored.The number of hair follicles per unit visual field was calculated in the treated back skin tissue of mice stained by HE.Serum levels of intercellular adhesion molecule-l(ICAM-1)and endothelium-leukocyte adhesion molecule-1(ELAM-1)were determined by enzyme-linked immu-nosorbent assay(ELISA).The contents of malondialdehyde(MDA)and superoxide dismutase(SOD)in skin tissue were determined by water-soluble tetrazole salt-1 method(WST-1).Results Compared with blank group,hair growth and hair score were significantly decreased in model group(P<0.05),the number of hair follicles was significantly decreased(P<0.05),serum ICAM-1 and ELAM-1 levels were increased(P<0.05),MDA content in skin tissue was increased(P<0.05),and SOD content was decreased(P<0.05).Compared with model group,hair growth and hair score were significantly increased in CeO2 nanoenzyme group(P<0.05),the number of hair follicles was significantly increased(P<0.05),serum ICAM-1 and ELAM-1 levels were decreased(P<0.05),MDA content in skin tissue was decreased(P<0.05),and SOD content was increased(P<0.05).Conclusion Topical CeO2 nanoenzymes has therapeutic effect on cyclophosphamide-induced alopecia areata of mice,which provides a theoretical basis for the external application of CeO2 nanoenzymes in the treatment of alopecia areata in the future.