ELF1 promotes EMT of stomach adenocarcinoma by targeting negative regulation of TRIM16
Objective To investigate the effect and mechanism of E74-like factor 1(ELF1)on migration,invasion and epithelial-mesenchymal transition(EMT)of stomach adenocarcinoma.Methods The expression of ELF1 in stomach adenocarcinoma tissue was analyzed using the gene expression profile interactive analysis 2(GEPIA2)database.The expression of ELF1 in gastric cancer cells was detected by RT-qPCR and Western blot.AGS cells were transfected with ELF1 short hairpin RNA(sh-ELF1)or its negative control short hairpin RNA(sh-NC),which was named as sh-ELF1 group and sh-NC group,respectively.The migration and invasion abilities were detected by wound healing assay and Transwell invasion assay,respectively.The expressions of EMT-related molecules,Snail,E-cadherin and Vimentin,and ELF1 target molecule tripartite motif containing 16(TRIM16)were analyzed by Western blot.Chromatin immunoprecipitation PCR(ChIP-PCR)was used to identify the binding of ELF1 to TRIM16 promoter region.For the rescue experiment,AGS cells were divided into sh-ELF1+si-TRIM16 group(co-transfected with sh-ELF1 and TRIM16 small interfering RNA)and sh-ELF1+si-NC group(co-transfected with sh-ELF1 and negative control small interfering RNA).The expressions of TRIM16,Snail,E-cadherin and Vimentin were analyzed by Western blot.The migration and invasion abilities were detected by wound healing assay and Transwell invasion assay,respectively.Results ELF1 was upregulated in stomach adenocarcinoma tissues and stomach adenocarcinoma cells(P＜0.05).Compared with sh-NC group,the cell migration and invasion abilities were decreased in sh-ELF1 group(P＜0.05).Compared with sh-NC group,the expressions of Snail and Vimentin were decreased,while the expressions of E-cadherin and TRIM16 were increased in sh-ELF1 group(P＜0.05).ChIP-PCR results showed that ELF1 binds to the promotor region of TRIM16,thereby inhibiting the expression of TRIM16.Rescue experiments demonstrated that compared with sh-ELF1+si-NC group,the expressions of Snail,Vimentin were increased,while E-cadherin was decreased in sh-ELF1+si-TRIM16 group(P＜0.05).Furthermore,compared with sh-ELF1+si-NC group,the cell migration and invasion abilities were increased in sh-ELF1+si-TRIM16 group(P＜0.05).Conclusion ELF1 can promote migration,invasion and EMT of stomach adenocarcinoma cells by negatively regulating of TRIM16.
万方数据
维普
