Inhibitory effect of intelligent curcumin nanoparticles combined with hyperthermia on hepatocellular carcinoma Huh 7 cells
Objective To prepare and characterize curcumin(Cur)-Fe3O4 nanoparticles(Cur-Fe3O4 NPs),and explore the inhibitory effect of NPs plus hyperthermia on the Huh 7 cells in vitro.Methods The Fe3O4 nanoclusters were synthesized by solvothermal method,and then chemically bonded with Cur to prepare the Cur-Fe3O4 NPs.NPs were verified by Fourier infrared spectroscopy(FT-IR)and transmission electron microscopy(TEM).The encapsulation efficiency of the NPs was determined by ultraviolet spectrophotometry.The drug release experiment in vitro was explored by dialysis method,and the Cur release rate was compared between radiofrequency hyper-thermia(RF)group and non-hyperthermia group.According to different treatment methods,the Huh 7 cells were divided into simple hyperthermia group,free Cur group,Cur-Fe3O4 NPs group,Cur+RF group and Cur-Fe3O4 NPs+RF group,and then the cytotoxicity was evaluated by CCK-8 assay.Results The Fe-0 bond was observed at 570 cm-1 and the Cur bond was successfully connected at 963.75 cm-1 on FT-IR.The TEM images showed that the sizes of Fe3O4 nanoclusters and Cur-Fe3O4 NPs were(50.7±10.2)nm and(94.0±12.2)nm,respectively.The encapsulation efficiency of NPs was 69.91％.The Cur-Fe3O4 NPs responsively released 68％of Cur in non-hyperthermia group,and the Cur release significantly increased to 85％in hyperthermia group.The survival rate of Huh 7 cells in simple hyperthermia group and free Cur group was about 90％.Compared with simple hyperthermia group and free Cur group,the cell viability was decreased in Cur-Fe3O4 NPs group(P＜0.05).Compared with the other three groups,the survival rate of Huh 7 cells was significantly reduced in Cur-Fe3O4 NPs+RF group(P＜0.000 1).Conclusion The Cur-Fe3O4 NPs can responsively release Cur,and significantly inhibit the Huh 7 cells after cooperated with hyperthermia.
hepatocellular carcinomacurcuminintelligent drug delivery systemmagnetic resonance imaginghyperthermia
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