Effect of estrogen on D-galactose-induced oxidative stress injury of HEI-OC1 cells
Objective To investigate the effect of estrogen on D-galactose-induced oxidative stress in cochlear hair cells.Methods The mouse cochlear hair cell line HEI-OC1 was cultured in vitro,and then divided into blank control group,D-galactose group(20 mg/mL)and estrogen+D-galactose group(20 mg/mL D-galactose+10 nmol/L β-estradiol).CCK-8 was used to detect the proliferation of HEI-OC1 cells in each group.DCFH-DA probe technology was used to detect ROS content in HEI-OC1 cells in each group.Superoxide dismutase(SOD)kit was used to detect the content of SOD in HEI-OC1 cells in each group.Western blot was used to detect the protein expression levels of nuclear factor E2-related protein(Nrf2)and heme oxygenase(HO-1)in each group.Results Compared with blank control group,the cell proliferation activity was decreased in D-galactose group(P＜0.01),ROS content was increased(P＜0.01),and SOD activity and Nrf2 and HO-1 protein expression levels were significantly decreased(P＜0.01).Compared with D-galactose group,the cell proliferation activity was increased in estrogen+D-galactose group(P＜0.01),ROS level was decreased(P＜0.01),and SOD activity and Nrf2 and HO-1 protein expression levels were significantly increased(P＜0.01).Conclusion Estrogen can alleviate the damage caused by D-galactose-induced oxidative stress in HEI-OC1 cells through Nrf2/HO-1 pathway.
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