Effects of geraniin on osteogenic differentiation of human periodontal ligament stem cells in inflammatory microenvironment
Objective To investigate the effect of geraniin(GER)on osteogenic differentiation and nuclear factor-κB(NF-κB)of human periodontal ligament stem cells(hPDLSCs)in inflammatory microenvironment.Methods The hPDLSCs isolated from human periodontal ligament tissue were divided into control group,tumor necrosis factor-α(TNF-α)group,0.1 μmol/L geraniin group(0.1GER group),1 μmol/L geraniin group(1GER group),10 μmol/L geraniin group(1OGER group)and 100 μmol/L geraniin group(100GER group).The hPDLSCs in control group were cultured in osteogenic induction medium,hPDLSCs in TNF-α group were cultured in osteogenic induction medium containing 10 ng/mL TNF-α,and hPDLSCs in 0.1GER group,1GER group,10GER group and 100GER group were cultured in osteogenic induction medium containing 10 ng/mL TNF-α and 0.1,1,10,100 μmol/L geraniin,respectively.At 21 d after culture,the activity of alkaline phosphatase(ALP)was detected by visible light colorimetry.The formation of calcified nodules was observed by Alizarin red staining.The relative expressions of Runt-related transcription factor 2(RUNX2),osteopontin(OPN),osteocalcin(OCN),osterix(OSX)and dentin sialophosphoprotein(DSPP)mRNA were detected by qRT-PCR.The phospho-rylation level of NF-κB p65 was detected by Western blot.Results Compared with control group,the relative ALP activity and the degree of calcified nodules were decreased in TNF-α group(P＜0.05),the relative mRNA expressions of RUNX2,OPN,OCN,OSX and DSPP were decreased(P＜0.05),while the relative phosphorylation level of NF-κB p65 was increased(P＜0.05).Compared with TNF-α group,the relative ALP activity and the degree of calcified nodules were increased in 1GER group,10GER group and 100GER group(P＜0.05),the relative mRNA expressions of RUNX2,OPN,OCN,OSX and DSPP were increased(P＜0.05),while the relative phosphorylation level of NF-κB p65 was decreased(P＜0.05).Conclusion Geraniin can improve the osteogenic differentia-tion of hPDLSCs in inflammatory microenvironment by inhibiting the activation of NF-κB.
万方数据
维普
