摘要
目的 建立高效液相色谱法同时测定香砂和胃丸中芸香柚皮苷、柚皮苷、橙皮苷、新橙皮苷、和厚朴酚以及厚朴酚的含量.方法 HPLC法色谱柱采用依利特SinoChrom ODS-BP C18柱(250 mm ×4.6 mm,5 μm);以0.2％磷酸水溶液(A)-乙腈(B)为流动相,梯度洗脱;流速1.0 mL/min;检测波长230 nm;进样量10 μL;柱温30 ℃.结果 芸香柚皮苷、柚皮苷、橙皮苷、新橙皮苷、和厚朴酚、厚朴酚分别在 3.274～32.740μg/mL(r=0.999 8)、6.882～68.820 μg/mL(r=0.999 9)、20.966～209.660 μg/mL(r=0.999 9)、5.754～57.540 μg/mL(r=0.999 9)、1.980～19.800μg/mL(r=0.999 9)、2.274～22.740 μg/mL(r=0.999 9)范围内线性关系良好;平均加样回收率分别为101.00％(RSD=0.71％)、101.92％(RSD=0.60％)、102.37％(RSD=0.58％)、98.05％(RSD=0.43％)、100.98％(RSD=0.81％)、100.64％(RSD=0.83％).结论 建立的 HPLC 法灵敏度高、操作简便、专属性好,可为香砂和胃丸的质量控制提供依据.
Abstract
Objective To establish an high-performance liquid chromatography(HPLC)method for the simultaneous determination of narirutin,naringin,hesperidin,neohesperidin,honokiol and magnolol in Xiangsha Hewei pills.Methods The separation was performed on an Elite Sino Chrom ODS-BP C18 column(250 mm ×4.6 mm,5 μm)with 0.2％phosphoric acid aqueous solution(A)-acetonitrile(B)as mobile phase for gradient elution.The flow rate was 1.0 mL/min,the detection wavelength was 230 nm,the injection volume was 10 μ.L,and the column temperature was 30 ℃.Results Narirutin,naringin,hesperidin,neohesperidin,honokiol and magnolol showed good linear relationships within the ranges of 3.274-32.740 μg/mL(r=0.999 8),6.882-68.820 μg/mL(r=0.999 9),20.966-209.660 μg/mL(r=0.999 9),5.754-57.540 μg/mL(r=0.999 9),1.980-19.800 μg/mL(r=0.999 9),2.274-22.740 μg/mL(r=0.999 9),respectively.The average recoveries were 101.00％(RSD=0.71％),101.92％(RSD=0.60％),102.37％(RSD=0.58％),98.05％(RSD=0.43％),100.98％(RSD=0.81％),100.64％(RSD=0.83％),respectively.Conclusion The established HPLC method has the advantages of high sensitivity,simple operation and good specificity,which can provide a basis for the quality control of Xiangsha Hewei pills.
基金项目
曲靖医学高等专科学校开放课题(2023YXYKF004)
维普
万方数据