Expression of ERα in hypoglossal nucleus in rats with different estrogen levels
Objective To study the effect of ovariectomy on the expression of estrogen receptor ERα in the hypoglossal nucleus of female rats.Methods Ninety 8-week-old female SD rats,weighing(250±25)g,were randomly and equally divided into sham ope-ration group(sham group),ovariectomized group(OVX group),and ovariectomized plus estradiol group(OVX+E2 group)to establish different estrogen level rat models.The rats in sham group were performed the removal of a small amount of fat around the ovary,the rats in OVX group were performed the removal of the ovary,and the rats in OVX+E2 group were subcutaneously injected with estradiol after ovariectomy.Immunohistochemistry was used to detect the expression of ERα in the hypoglossal nucleus.Real-time PCR and Western blot were used to detect the expressions of ERα mRNA and protein.Results ERα showed positive expression in the hypo-glossal nucleus of rats in all three groups.Compared with sham group,the mRNA and protein expressions of ERα were significantly reduced in OVX group(P＜0.01).Compared with OVX group,the mRNA and protein levels of ERα were significantly increased in OVX+E2 group(P＜0.01).There was no statistically significant difference in ERα expression between sham group and OVX+E2 group(P＞0.05).Conclusion Estrogen receptor ERα is expressed in the hypoglossal nucleus of female rats with different estrogen levels,and the levels of ERα mRNA and protein are regulated by estrogen level.
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