HIF-1α involves in the development of periodontitis by regulating type H vessels
Objective To investigate whether hypoxia inducible factor-1α(HIF-1α)and type H vessels are involved in the periodontitis microenvironment.Methods Ten C57BL/6 mice were randomly divided into periodontitis group and control group,with 5 mice in each group.The maxillary second molar of mice in periodontitis group were ligated with 5-0 silk thread to establish the experimental periodontitis model,while the mice in control group were not treated.After ligation for 14 d,the changes of alveolar bone height of the maxillary second molars were detected by micro-CT scanning,the expression of cathepsin K(CTSK)was analyzed by immunohistochemistry,and the formation of type H vessels(CD31+Emcn+)and the expression of HIF-1α in periodontal tissues were analyzed by immunofluorescence analysis.Results The experimental periodontitis model was successfully established by silk thread ligature.The micro-CT scanning results showed that the distance between the cementoenamel junctions and the alveolar bone crest(CEJ-ABC)of mice in periodontitis group was significantly higher than that in control group(P＜0.001),and HE staining results demonstrated that the sevious inflammatory pathological reaction was observed in periodontitis group.Immunohistochemical analysis indicated that CTSK expression level was significantly higher in periodontitis group than in control group(P＜0.05).Immunofluores-cence analysis demonstrated that HIF-1α expression level was increased in periodontitis group compared with control group(P＜0.000 1),and the number of type H vessels was also significantly increased in periodontitis group,as evidenced by higher densities of CD31+Emcn+(P＜0.001).Conclusion The mouse periodontitis model is successfully established by silk ligation,and the expressions of HIF-1α and type H vessels are specifically elevated in the periodontal tissues within periodontitis,which suggests that HIF-1α and type H vessels may be involved in the process of bone resorption in periodontitis alveolar bone.
periodontitisHIF-1αCTSKtype H vesselsbone resorption
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