Identification of primary components and assay of amino acids in Astragalus injections
Objective To identify the primary components in Astragalus injection and evaluate the quality differences of Astragalus injections from different manufacturers based on the contents of amino acids.Methods The primary components in Astragalus injec-tion were detected by gradient elution using 0.1％ acetonitrile formate and 0.1％ formic acid water as mobile phase by LC-MS/MS method.With 10％methanol water(containing 0.1％ formic acid)and 50％ methanol water(containing 0.1％ formic acid)as mobile phases,the content of each amino acid was determined after gradient elution,and the quality difference of amino acids in the primary components of Astragalus injections from different manufacturers was evaluated by principal component analysis.Results A total of 255 primary components were detected in all Astragalus injections from different manufacturers,including amino acids,organic acids,sugars and N-containing compounds.And the score of amino acid content in four manufacturers was-0.23,-1.77,-0.35,2.36,respectively.Conclusion The types of primary components in Astragalus injection from different manufacturers are the same,but the contents of primary components are different.The contents of amino acids in Astragalus injections from different manufacturers vary greatly.
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