Correlation of vitamin D receptor gene polymorphisms with cervical lesions and regression of HPV infection
Objective To analyze the relationship between vitamin D receptor(VDR)gene polymorphisms and cervical lesions,and explore VDR gene polymorphism on the progression of high-risk human papillomavirus(HR-HPV)infection.Methods A total of 376 patients who underwent colposcopy in the Second Hospital of Shanxi Medical University from September 2020 to October 2021 were selected.According to pathological results,the patients were divided into control group(188 cases of chronic cervicitis and CIN Ⅰ)and case group(188 cases of CIN Ⅱ or above).Peripheral venous blood samples from the 376 patients were collected for VDR gene polymorphisms,including four single nucleotide polymorphisms(SNPs)sites:FOKI,BsmI,ApaI,and TaqI,and the relationships between VDR gene polymorphisms and cervical lesions were analyzed.Among the 142 patients who underwent cervical conization for CIN Ⅱ-Ⅲ,the outcomes of HR-HPV infection were followed up for 6 months.Based on the follow-up results,the patients were divided into two groups:clearance group(HPV negative)and persistent infection group(persistent infection with the same HPV type),and the impact of VDR gene polymorphisms on the outcome of HPV infection was analyzed.Results The follow-up results showed that HPV infection became negative in 104 patients,33 patients continued to be infected with the same type of HPV,and 5 patients were infected with other types of HPV.The FOKI SNPs ff genotype and f allele were significantly associated with the occurrence of CINⅡ+(P＜0.05),and also related to persistent high-risk human papillomavirus(HR-HPV)infection(P＜0.05).The TaqI SNPs tt and Tt genotypes increased the risk of CIN Ⅱ+occurrence(P＜0.05),and the t allele was associated with an increased risk of CIN Ⅱ+(P＜0.05),but not with persistent HPV infection(P＞0.05).Conclusion VDR gene polymorphisms are associated with cervical lesions and the outcome of HR-HPV infection.Specifically,the FOKI SNPs ff genotype and f allele,and the TaqI SNPs tt and Tt genotypes and t allele are associated to the occurrence of cervical lesions.Furthermore,the FOKI SNPs ff genotype and f allele may influence the outcome of HR-HPV infection.
human papillomavirusvitamin D receptorgenetic polymorphismcervical lesionsTaqman gene probesingle nucleotide polymorphism
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