Preparation and preliminary evaluation in vitro of resveratrol nanosuspensions
Objective To prepare and in vitro evaluate the resveratrol(RES)nanosuspension.Methods RES nanosuspensions were prepared by precipitation method.With the average particle size and the polydispersity index(PDI)as evaluation indexes,the orthogonal test was used to optimize the formulation process.The freeze-dried powder of nanosuspension was prepared by freeze-drying method and characterized.The in vitro release of the preparation was studied by dialysis bag method.The ARPE-19 cell injury model was estab-lished by high glucose stimulation,and the effect of RES nanosuspension on the cell viability was evaluated by CCK-8 method.Results The optimal prescription process of RES was as follows:the concentration of RES was 6 mg/mL,the mass ratio of two stabilizers(PVP K17∶HPMC)was 2∶1,and the mass ratio of RES and stabilizer was 1∶2,and 5％mannitol was freeze-dried protective agent.The RES nanosuspension was relatively regular granule,with a drug loading of 28.04％and a cumulative release of 91.27％within 36 h.Compared with RES,RES nanosuspension pretreatment significantly increased the viability of ARPE-19 cells damaged by high glucose.Conclusion RES nanosuspension could increase the release rate of RES in vitro,and could attenuate the damage of ARPE-19 cells by high glucose and improve the cell survival rate.
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