Effect of Hewei Jiangni capsules on apoptosis of gastric adenocarcinoma cells and its mechanism
Objective To observe the effects of serum containing Hewei Jiangni on proliferation and apoptosis of gastric cancer cells(AGS)and its mechanism based on NF-κB/EMT signaling pathway.Methods Serums containing low,medium,and high concen-trations of Hewei Jiangni(3,6,and 12 g/kg)were prepared,and the blank serum was used as control group.AGS cells were intervened with serums containing different concentrations of Hewei Jiangni for 24,48,72 h respectively.The proliferation of AGS cells was detected by CCK-8 kit.The apoptosis of AGS cells was detected by DAPI staining.The migration of AGS cells was observed by Scratch test.The mRNA levels of NF-κB and EMT marker proteins E-cadherin and Vimentin were detected by quantitative real-time polymerase chain reaction(RT-qPCR).The protein expressions of NF-κB,E-cadherin,and Vimentin were detected by Western blot.Results At the same time points,compared with control group,the viability of cells was dose-dependently decreased in Hewei Jiangni groups(P＜0.01).Compared with 24 h,the viability of AGS cells was decreased in Hewei Jiangni groups at 48,72 h(P＜0.05).At 48 h,compared with control group,the apoptosis of AGS cells was dose-dependently increased in Hewei Jiangni groups(P＜0.05).Compared with control group,the scratch healing speed was slowed down,and the rate of cell migration was reduced in Hewei Jiangni groups(P＜0.05).Compared with control group,the migration was decreased at 24 h in high dose group(P＜0.01),and was also reduced at 48 h in medium and high dose groups(P＜0.01).Compared with control group,the mRNA levels of NF-κB and Vimentin in AGS cells were dose-dependently downregulated in Hewei Jiangni groups(P＜0.05),while E-cadherin mRNA was dose-dependently upregulated(P＜0.01).Compared with control group,the protein levels of NF-κB and Vimentin in AGS cells were dose-dependently downregulated while the E-cadherin proein expression was dose-dependently upregulated in Hewei Jiangni groups(P＜0.05).Conclusion Hewei Jiangni can mediate the migration and invasion of AGS cells by inhibiting the activation of NF-κB,and regulating the expressions of downstream EMT-related proteins,thus inhibiting the proliferation and promoting the apoptosis of AGS cells.
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