FUT6 promotes the proliferation,invasion and migration of gastric cancer cells
Objective To investigate the expression of α1,3 fucosyltransferase-Ⅵ(FUT6)in gastric cancer and its effects on the pro-liferation,invasion and migration of gastric cancer cells.Methods GEPIA website and The Cancer Genome Atlas(TCGA)database were used to investigate the expression of FUT6 in gastric cancer and analyze its relationship with tumor immunity.The protein expres-sion level of FUT6 in gastric cancer cell lines(MKN-45,MGC-803,BGC-823,SGC-7901,AGS)and gastric mucosal epithelial cell line(GES-1)was detected by Western blot to screen the cells with the highest FUT6 expression for the further experiments.SGC-7901 cells with low FUT6 expression were constructed by transient transfection.The cell proliferation ability was detected by CCK-8 method,the cell invasion and migration abilities were detected by Transwell assay,the cell migration ability was detected by scratch assay,and the cell transfection efficiency and the expressions of epithelial mesenchymal transition(EMT)-related proteins(E-cadherin,N-cadherin,Vimentin)were detected by Western blot.Results The expression of FUT6 was abnormal in various cancers,and the expression of FUT6 in gastric cancer was significantly increased(P<0.05).The expression of FUT6 in gastric cancer tissues was significantly higher than that in adjacent tissues(P<0.05).The expression of FUT6 was correlated with immune cells,immunostimulators,immunosup-pressors,chemokines,chemokine receptors and immune checkpoint inhibitors in gastric cancer(P<0.05).FUT6 expression was significantly higher in gastric cancer cells(MKN-45,MGC-803,BGC-823,SGC-7901 and AGS)than in GES-1 cells(P<0.05).After FUT6 knockdown,the proliferation rate of SGC-7901 cells was significantly decreased(P<0.05),the cell migration distance was decreased(P<0.05),the expression of E-cadherin protein was increased(P<0.05),and the expressions of N-cadherin and Vimentin proteins were decreased(P<0.05).Conclusion FUT6 is highly expressed in gastric cancer and participates in the immune microen-vironment of patients with gastric cancer.Knockdown of FUT6 can inhibit the proliferation,invasion and migration of gastric cancer cells,which may be related to EMT.
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