Research and quality evaluation on fingerprint of Baohe pills by HPLC
Objective To establish a fingerprint of Baohe pills for providing references in the effective quality control and the scien-tific evaluation.Methods The common peaks of the fingerprint spectrum were calibrated on Kromasil C18 column(4.6 mm×250 mm,5 μm)with Acetonitrile and 0.5%glacial acetic acid as the mobile phase for gradient elution at a flow rate of 0.7 mL/min at the detection wavelength of 320 nm.Taking hesperidin as the reference peak,the similarity of 244 batches of different dosage forms of Baohe pills from 61 production managements was evaluated by the software of Similarity Evaluation System for Chromatographic Fingerprint of TCM(version 2012).Results A total of 7 common peaks were identified by the established HPLC fingerprint of Baohe pills,including charred fructus crataegi(peaks 1,2),radish seed(peaks 3,6),forsythia(peak 4),forsynoidide A(peak 5)and hesperidin(peak 7).The similarity of common peaks of 39 batches of water pills was above or equal to 0.90,and that of 17 batches was below 0.90.The simi-larity of the common peaks of 56 batches of big honeyed pills was above or equal to 0.90,and that of 25 batches of samples was below 0.90.The similarity of common peaks in 56 batches of concentrated pills was above or equal to 0.90,and that of 51 batches was below 0.90.Conclusion The established HPLC fingerprint method is simple and fast,with good reproducibility,and can exhibit the overall characteristics of multiple components in Baohe pills,which provides a scientific basis for the quality control of Baohe pills.
国家科技期刊平台
NSTL
万方数据
