Rab3A is involved in myocardial ischemia/reperfusion injury by regulating the aberrant expression of integrin subunit α3
Objective:To investigate the role of G protein Rab3A and integrin subunit α3(ITGA3)in the process of myocardial ischemia/reperfusion injury and the potential molecular mechanism.Methods:The model of myocardial ischemia/reperfusion injury was established by hypoxia/reoxygenation method;The expression level of Rab3A in myocardial cells was detected by real-time quantitative polymerase chain reaction(RT-qPCR)and western blot.Cell counting kit-8(CCK-8)and apoptosis detection kits were used to assess the effects of Rab3A on cardiomyo-cyte viability and apoptosis,and the commercial kits were used to detect the effect of Rab3A on oxidative stress in cardiomyocytes.The dual luciferase assay was used to detect the binding of Rab3A to ITGA3.Results:The expression of Rab3A mRNA and protein in cardiomyocytes induced by hypoxia/reoxygenation was significantly down-regulated(P＜0.05).Overexpression of Rab3A reversed the decrease in cell viability and increase in apoptosis level induced by ischemia/reperfusion injury(all P＜0.05),as well as overexpression of Rab3A inhibited the oxidative stress induced by ischemia/reperfusion injury(P＜0.05).There is a targeted binding site between Rab3A and ITGA3,and Rab3A can regulate the expression of ITGA3.Conclusion:Rab3 A can affect the proliferation,apoptosis and oxidative stress of cardiomyocytes by regulating the abnormal expression of ITGA3,and participate in the process of myocardial ische-mia/reperfusion injury.
万方数据
维普
