Expression of circular RNA circ-SLAIN2 in serum of patients with acute myocarditis and its effect on myocardial cell activity
Objective:To observe the expression of circular RNA(circRNA)circ-SLAIN2 in serum of patients with acute myocarditis,and to explore the effect of circ-SLAIN2 on myocardial cell proliferation and apoptosis and its mechanism.Methods:Serum samples from 35 patients with acute myocarditis treated in the Department of Cardiovas-cular Medicine,Jingmen People's Hospital from July 2020 to May 2023 were collected,and 35 healthy individuals were selected as controls.The expression of circ-SLAIN2 in serum was detected by quantitative real-time polymerase chain reaction(qRT-PCR).The human cardiomyocyte cell line HCM was selected and infected with empty lentivirus(Control group)or lentivirus loaded with circ-SLAIN2 sequence(circ-SLAIN2 group).The overexpression efficiency of circ-SLAIN2 was detected by qRT-PCR.Myocarditis was induced by lipopolysaccharide,and the effects of circ-SLAIN2 overexpression on the proliferation and apoptosis of human cardiomyocytes were detected by Cell Counting Kit-8(CCK-8)assay and flow cytometry.The LncBase v.2,Linc2GO,LNCipedia,miRcode online database and dual-luciferase reporter gene experiments were used to analyze the targeting relationship between circ-SLAIN2 and miR-429.The expression level of miR-429 was detected by qRT-PCR.Western blot was used to detect the expression lev-els of Mitogen-activated protein kinase p38(p38MAPK)/nuclear factor-kappa B(NF-κB)signaling pathway pro-teins and apoptosis-related proteins.Results:The expression of circ-SLAIN2 in serum of patients with acute myocar-ditis was significantly lower than that of healthy subjects(P＜0.01).Compared with the control group,the expres-sion of circ-SLAIN2 in HCM cells in the circ-SLAIN2 group was significantly increased(P＜0.01).After lipopo-lysaccharide treatment for 36 h,the absorbance of HCM cell proliferation in circ-SLAIN2 group was significantly higher than that in Control group(P＜0.01),and the apoptosis rate of HCM cells in circ-SLAIN2 group was signifi-cantly lower than that in Control group(P＜0.01).circ-SLAIN2 can target and bind miR-429.Compared with the control group,the expression of miR-429 in HCM cells in the circ-SLAIN2 group was significantly decreased(P＜0.01),the expression of p38MAPK/NF-κB signaling pathway proteins was significantly decreased(P＜0.01),and the expression of apoptosis proteins Bcl-2 associated X protein(Bax),Bcl-2 associated K protein(Bak)was decreased(all P＜0.01).Conclusion:circ-SLAIN2 is lowly expressed in the serum of patients with acute myocarditis,and over-expression of circ-SLAIN2 negatively regulates the expression of miR-429,enhances the activity of cardiomyocytes and inhibits cell apoptosis.
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