摘要
目的:本研究旨在探讨富血小板血浆微球对宫颈癌根治术后尿失禁大鼠尿动力学及阴道前壁胶原代谢影响.方法:将60只6～8周未生产的雌性大鼠按随机数字表法分为正常组、模型组、富血小板血浆组、空白明胶微球组及富血小板血浆微球组各12只,制备宫颈癌根治术后尿失禁大鼠模型成功后,正常组、模型组正常饲养,其余三组依次采用微量注射器注入富血小板血浆、空白明胶微球及富血小板血浆微球.观察各组大鼠一般情况、尿动力指标、膀胱内压、尿道括约肌组织形态、阴道前壁转化生长因子-β(TGF-β)、金属蛋白酶组织抑制剂-1(TIMP-1)、基质金属蛋白酶-1(MMP-1)、基质金属蛋白酶-9(MMP-9)、Ⅰ型胶原蛋白(Col-Ⅰ)信使核糖核酸(mRNA)表达水平、TGF-β、TIMP-1、MMP-1、MMP-9、Col-Ⅰ蛋白表达.结果:与模型组比较,正常组、富血小板血浆组、空白明胶微球组及富血小板血浆微球组尿动力指标、膀胱内压、阴道前壁TGF-β mRNA/蛋白、TIMP-1 mRNA/蛋白、MMP-1 mRNA/蛋白、MMP-9 mRNA/蛋白、Col-Ⅰ mRNA/蛋白均改善(均P＜0.05);与富血小板血浆组、空白明胶微球组比较,正常组及富血小板血浆微球组尿动力指标、膀胱内压、阴道前壁TGF-β mRNA/蛋白、TIMP-1 mRNA/蛋白、MMP-1 mRNA/蛋白、MMP-9 mRNA/蛋白、Col-Ⅰ mRNA/蛋白均改善(均P＜0.05);与富血小板血浆微球组比较,正常组尿动力指标、膀胱内压、阴道前壁 TGF-β mRNA/蛋白、TIMP-1 mRNA/蛋白、MMP-1 mRNA/蛋白、MMP-9 mRNA/蛋白、Col-Ⅰ mRNA/蛋白均改善(均P＜0.05);富血小板血浆组与空白明胶微球组比较,差异有统计学意义(均P＞0.05).结论:富血小板血浆微球能够恢复宫颈癌根治术后尿失禁大鼠尿动力学,同时能够调节胶原代谢.
Abstract
Objective:To investigate the effects of platelet-rich plasma microspheres on urodynamics and colla-gen metabolism of anterior vaginal wall in rats with urinary incontinence after radical cervical cancer surgery.Methods:A total of 60 female rats that had not given birth for 6 to 8 weeks were divided into normal group,model group,platelet-rich plasma group,blank gelatin microsphere group and platelet-rich plasma microsphere group with 12 rats in each group according to random number table method.After successful preparation of urinary incontinence rat model after radical cervical cancer surgery,normal group and model group were fed normally,and the other three groups were injected with platelet-rich plasma,blank gelatin microspheres and platelet-rich plasma microspheres with microsyringes.The general condition,urinary dynamic indexes,internal bladder pressure,urethral sphincter tissue morphology,anterior vaginal wall transforming growth factor-β(TGF-β),tissue inhibitor of metalloproteinase-1(TIMP-1),matrix metalloproteinase-1(MMP-1),matrix metalloproteinase-9(MMP-9),type Ⅰ collagen(Col-ⅠmRNA expression level,TGF-β,TIMP-1,MMP-1,MMP-9,Col-Ⅰ protein expression.were observed in each group.Results:Compared with the model group,urinary motility indexes,bladder internal pressure,TGF-β mRNA and pro-tein,TIMP-1 mRNA and protein,MMP-1 mRNA and protein,MMP-9 mRNA and protein,Col-Ⅰ mRNA and pro-tein in normal group,platelet-rich plasma group,blank gelatin microsphere group and platelet-rich plasma micro-sphere group mRNA and protein were improved(all P＜0.05).Compared with platelet-rich plasma group and blank gelatin microsphere group,urinary motility index,bladder internal pressure,TGF-β mRNA and protein,TIMP-1 mRNA and protein,MMP-1 mRNA and protein,MMP-9 mRNA and protein,Col-Ⅰ mRNA and protein were im-proved in normal group and platelet-rich plasma micropellet group(all P＜0.05).Compared with platelet-rich plas-ma micropellet group,urinary motility index,bladder pressure,TGF-β mRNA and protein,TIMP-1 mRNA and pro-tein,MMP-1 mRNA and protein,MMP-9 mRNA and protein,Col-Ⅰ mRNA and protein in normal group were im-proved(all P＜0.05).The above indexes in platelet-rich plasma group were not different from blank gelatin micro-sphere group(all P＞0.05).Conclusion:Platelet-rich plasma microspheres can restore urodynamics and regulate col-lagen metabolism in rats with urinary incontinence after radical cervical cancer surgery.
维普
万方数据