Study on the Mechanism of Hedyotis Diffusa in Treating Acute Myeloid Leukemia Based on Network Pharmacology and Molecular Docking Technology
Objective To investigate the possible molecular mechanisms of Hedyotis diffusa in treating acute myeloid leukemia(AML)through network pharmacology and molecular docking techniques.Methods The TCMSP and PubChem databases were used to collect the components and targets of compounds related to Hedyotis diffusa,and the GeneCards and OMMI databases were used to select the relevant targets that have been proven to be related to acute leukemia.Then,the intersection was taken to screen for common genes,and the"drug,component,disease,target"relationship graph was constructed by importing it into Cytoscape software.The intersection targets were then input into the STRING analysis platform to draw a PPI map;Afterwards,GO and KEGG process analysis were performed on key targets using R software.Using AutoDock software package for molecular docking,and finally analyzing the expression and prognosis of core genes in AML through GEPIA database.Results There were 82 possible target genes of Hedyotis diffusa in the treatment of AML,involving multiple processes such as response to heterogeneous stimuli,regulation of body fluid level,wound healing,and closely related to biological processes such as chemical carcinogenesis receptor activation,lipid and related atherosclerosis,fluid shear stress,and hepatitis B.Hedyotis diffusa may treat AML through molecular functions such as DNA binding transcription factor activation,RNA polymerase Ⅱ specific DNA binding,and other processes such as response to heterogeneous stimuli,regulation of humoral level,and wound healing,and is closely related to chemical carcinogenesis related receptor activation and arterial Congee related pathways.Five genes,including IL6,HSP90AA1,HIF1A,CASP3,and ESR1,were upregulated in AML,with significant differences in the expression of IL6 and HIF1A;The ESR1 gene has a profound impact on the prognosis of patients(P＜0.01).Conclusion Preliminary research has revealed that Hedyotis diffusa exerts its therapeutic effect on AML in a complex form of multiple components,targets,and pathways,providing a theoretical basis for its clinical application.
万方数据
维普
