Research on Optimization of Extraction Process and Quality Standards of Tiaogan Huoxue Wenya Granules Based on Box-Behnken Design-Response Surface Methodology
Objective To optimize the extraction process of Tiaogan Huoxue Wenya granules and study its quality standards.Methods In this study,the comprehensive scores of extract yield and transfer rate of salvianolic acid B were used as indicators.Single factor investigation was combined with Box-Behnken design-response surface methodology to optimize the extraction process of Tiaogan Huoxue Wenya granules.Thin layer chromatography was used to identify the Chinese herbal medicine.Results The final extraction process was determined as follows:adding 10 times the amount of water and boiling for 90 minutes for the first time,and adding 6 times the amount of water and boiling for 60 minutes for the second time.The measurement method yields accurate and reliable results.Thin layer chromatography was used to detect characteristic spots in three traditional Chinese medicines:Tianma,Zhizi,and Danshen.Conclusion The optimized extraction process has simple operation,good extraction effect,and stable and reliable results.
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