The Effect of LncRNA Haglros Regulating miR-26b/JAK2/STAT3 Pathway on Epithelial Mesenchymal Transition of Hepatocellular Carcinoma Cells
Objective To explore the regulation of Long non-coding RNA(LncRNA)HAGLR complementary chain Ln-cRNA(HAGLROS)on microrNA(miRNA,miR)-26b/non-receptor protein tyrosine Kinase 2,Effect of JAK2/signal transducer and activator of transcription3(STAT3)pathway on epithelial mesenchymal transformation of hepatoma cells.Methods Normal liver cell lines and liver cancer cell lines were obtained and divided into si-NC group,si-HAGLROS group,miR-26 inhibitor group and si-HAGLROS +miR-26 inhibitor group according to transfection conditions.Cell proliferation activity was detected by MTT assay,cell migration was detected by cell scratch assay,cell invasion assay was detected by cell invasion assay,and HAGLROS,miR-26b,E-Cadherin(E-cad)and N-cadherin(N-cadherin)were detected by qPCR.E-cad),Fibronectin(FN),anti-apoptotic protein(Bcl-2)expression,dual luciferase reporter gene assay verified the targeting relationship between LncRNA-HAGLROS and miR-26b.Results The expression of HAGLROS in Hep3B,MHCC-LM3,Huh7 and SMMC-7721 cells was significantly higher than that in LO2 cells(P<0.05),while the expression of miR-26b was the opposite.The bioinformatics software showed that HA-GLROS could target miR-26b.Liver cancer cell function experiment showed that compared with si-NC group,cell proliferation ac-tivity,cell scratch healing rate,cell membrane crossing number,N-cadherin,FN,Bcl-2,p-JAK2,p-STAT3 expression were lower in si-HAGLROS group,while E-Cadherin expression was higher.However,in miR-26b inhibitor group,cell proliferation activity,cell scratch healing rate,cell membrane crossing number,N-cadherin,FN,Bcl-2,p-JAK2,p-STAT3 expression were higher,while E-Cadherin expression was lower.There was no statistical significance between si-NC group and si-HAGLROS + miR-26b inhibitor group(P>0.05).Conclusion LncRNA-HAGLROS can promote the biological behavior of liver cancer cells,such as migration,invasion and EMT process,by targeting down-regulation of miR-26b,and can regulate the activity of JAK2/STAT3 pathway.Ln-cRNA-HAGLROS can be used as a therapeutic target for liver cancer.
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